Tiron is widely used as a chelating agent in various biochemical and analytical applications. It is particularly effective in binding and stabilizing metal ions, such as iron and copper, which makes it valuable in studies involving oxidative stress and metal ion-mediated reactions. In biological research, Tiron is employed to scavenge reactive oxygen species (ROS) and protect cells from oxidative damage, making it a useful tool in understanding cellular mechanisms related to aging, inflammation, and disease. Additionally, Tiron is utilized in environmental science to remove heavy metals from contaminated water, aiding in pollution control and remediation efforts. Its ability to form stable complexes with metal ions also finds applications in industrial processes, such as metal extraction and purification.