Tiron is widely used as a chelating agent in various scientific and industrial applications. It is particularly effective in binding and sequestering metal ions, such as iron and aluminum, making it useful in studies involving metal ion detection and analysis. In biological research, Tiron is employed to study oxidative stress due to its ability to scavenge reactive oxygen species (ROS) and protect cells from oxidative damage. It is also utilized in environmental science to mitigate the effects of metal contamination in soil and water. Additionally, Tiron finds application in analytical chemistry as a reagent for colorimetric assays to determine metal ion concentrations. Its antioxidant properties further make it a candidate for research in pharmacology and toxicology.