Used as a pH indicator in acid-base titrations, turning red below pH 4.4 and yellow above pH 6.2. Commonly applied in microbiology for the methyl red test to detect mixed acid fermentation by bacteria, especially in identifying Enterobacteriaceae. Also utilized in analytical chemistry for endpoint determination in titrations involving weak bases and strong acids. Suitable for use in culture media and biochemical assays where precise pH monitoring is required.