Used as a pH indicator in acid-base titrations, particularly for the determination of total alkalinity in water samples containing carbonates and bicarbonates. The mixture provides a sharp color change from violet to green around pH 5.0–5.4 at the endpoint, making it effective for titrations of weak bases with strong acids. Commonly employed in environmental testing, water quality analysis, educational laboratories, and industrial quality control in sectors like food and beverages. Also utilized in microbiology for biochemical tests to detect pH changes due to microbial metabolism.