Used as a pH indicator in laboratory and industrial applications, particularly in titrations involving weak bases and strong acids. It changes color from red (below pH 4.4) to yellow (above pH 6.2), making it effective for detecting the endpoint in acid-base reactions. Commonly used in microbiology for methyl red tests to identify bacteria that produce stable acids from glucose fermentation, such as in the differentiation of Enterobacteriaceae. Also applied in environmental testing and quality control processes where precise pH monitoring in the acidic range is required.