Used as a fluorescent tracer in biological and medical research, particularly for labeling and tracking cells. It readily enters cells and binds to intracellular components, emitting bright green fluorescence when excited by light, making it useful in viability assays and cell migration studies. Commonly applied in bone research, where it labels actively mineralizing tissues, allowing visualization of bone growth and repair in animal models. Also utilized in membrane permeability assays and to assess the integrity of cellular barriers. Its low toxicity and strong fluorescence make it suitable for live-cell imaging and dual-labeling experiments with other fluorophores.