Widely used as a pH indicator in laboratory settings, particularly in electrophoresis. It changes color from yellow to blue as the pH increases, making it ideal for monitoring the progress of agarose and polyacrylamide gel electrophoresis. Its visible color shift helps in tracking the migration of nucleic acids or proteins during separation. Also used as a component in microbiological media to indicate pH changes due to metabolic activity. Commonly included in sample loading buffers to provide visual confirmation of sample loading and buffer front movement.