Used as a pH indicator in analytical chemistry, particularly in titrations and colorimetric assays. It changes color from yellow to blue over the pH range of 3.8 to 5.4, making it suitable for detecting weak acids and bases. Commonly employed in microbiology for bacterial differentiation in culture media, and in environmental testing to monitor pH changes in water and soil samples. Also utilized in educational labs for demonstrating acid-base reactions due to its clear and distinct color transition.