Used as a pH indicator in laboratory applications, particularly in electrophoresis gels to monitor the progress of migration. It changes color from yellow to blue as the pH increases, making it useful for visual tracking during DNA, RNA, or protein gel electrophoresis. Also serves as a tracking dye due to its distinct color and consistent migration rate in agarose and polyacrylamide gels. Commonly included in loading buffers to help visualize sample entry into the gel wells.