Pyrogallol Red is widely used as a pH indicator in various analytical and biochemical applications. It is particularly effective in detecting pH changes in the range of 6.5 to 8.5, making it suitable for monitoring neutral to slightly alkaline conditions. In biochemistry, it serves as a reagent for protein assays, where it binds to proteins and produces a color change that can be measured spectrophotometrically. This property is utilized in quantifying protein concentrations in biological samples. Additionally, Pyrogallol Red is employed in the determination of metal ions, such as aluminum and iron, due to its ability to form colored complexes with these metals. It is also used in environmental studies to analyze water quality by detecting trace metals. Its sensitivity and specificity make it a valuable tool in both laboratory research and industrial processes.