6-Nitroindazole is widely used in biochemical research as a selective inhibitor of neuronal nitric oxide synthase (nNOS). It helps in studying the role of nitric oxide in various physiological and pathological processes, such as neurotransmission, inflammation, and neurodegeneration. Additionally, it is utilized in pharmacological studies to explore potential therapeutic interventions for conditions like stroke, Parkinson's disease, and chronic pain, where nNOS activity is implicated. Its ability to modulate nitric oxide pathways makes it a valuable tool in understanding cellular signaling mechanisms.