Trehalose 6,6′-dimycolate is primarily utilized in immunological research due to its role as a key component of the cell wall in certain mycobacteria, particularly Mycobacterium tuberculosis. It is known to stimulate the immune system by interacting with macrophages and dendritic cells, leading to the production of cytokines and the activation of T-cells. This makes it a valuable tool in studying host-pathogen interactions and developing vaccines. Additionally, it is used in adjuvant research to enhance the immune response to various antigens, potentially improving the efficacy of vaccines. Its unique structure and immunomodulatory properties also make it a subject of interest in cancer immunotherapy, where it is explored for its ability to modulate the tumor microenvironment and boost anti-tumor immunity.