Fluorescein tyramide is widely used in molecular biology and immunohistochemistry for signal amplification in detection assays. It is a key component in the tyramide signal amplification (TSA) technique, which enhances the sensitivity of detecting low-abundance targets in tissue samples. When combined with horseradish peroxidase (HRP), fluorescein tyramide is converted into a reactive intermediate that binds covalently to nearby proteins, producing a strong fluorescent signal. This makes it particularly useful for visualizing specific proteins, nucleic acids, or other biomolecules in complex biological samples. Its high sensitivity and compatibility with fluorescence microscopy have made it a valuable tool in research applications, such as studying gene expression, protein localization, and cellular processes in both fixed and live cells.