Antigen Retrieval Reagent is widely used in immunohistochemistry (IHC) to enhance the detection of antigens in tissue samples. It is particularly valuable when working with formalin-fixed, paraffin-embedded tissues, as the fixation process can mask or alter antigen epitopes, making them less accessible to antibodies. By applying this reagent, the antigen-antibody binding is restored, improving the accuracy and intensity of staining. This is critical for diagnosing diseases, such as cancer, where identifying specific biomarkers in tissue samples is essential. Additionally, it is used in research to study protein expression and localization in various biological contexts. The reagent is often employed in automated staining systems, ensuring consistency and efficiency in high-throughput laboratories.