ELISA kit is widely used in medical and biological research for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones. It is commonly applied in disease diagnosis, including infectious diseases like HIV, hepatitis, and more recently, SARS-CoV-2. The kit enables high-throughput screening in clinical labs and research settings due to its sensitivity and specificity. It is also used in food industry testing to detect allergens or contaminants. In pharmaceutical development, ELISA kits help monitor drug levels and immune responses during trials. Their ease of use and reliable results make them essential in both laboratory and diagnostic environments.