Glyoxal-bis-2,4-DNPH is a stable hydrazone derivative formed from glyoxal and 2,4-dinitrophenylhydrazine (DNPH). It serves primarily as a reference standard in analytical chemistry for the detection and quantification of dicarbonyl compounds, especially glyoxal, in environmental and biological samples. This compound is used in derivatization-based methods, where it acts as an internal or external standard for accurate analysis via techniques such as HPLC, LC-MS, or UV-Vis spectroscopy. Its applications include atmospheric pollution monitoring, assessment of oxidative stress in biomedical research, food safety evaluation, and industrial quality control for material degradation pathways.