DL-Pyroglutamic acid is widely used in the cosmetic industry as a moisturizing agent due to its ability to enhance skin hydration and improve the skin barrier function. It is often incorporated into skincare products like creams, lotions, and serums to help maintain skin elasticity and reduce dryness. It is also used in hair care products to strengthen hair and prevent breakage.

In the pharmaceutical field, it serves as an intermediate in the synthesis of various drugs, particularly those targeting neurological disorders, as it plays a role in neurotransmitter regulation.

Additionally, it is utilized in biochemical research as a precursor for studying peptide synthesis and protein folding mechanisms.

Its chelating properties also make it useful in certain industrial applications, such as metal ion stabilization in chemical processes.