Lacto-N-tetraose is primarily utilized in the field of infant nutrition due to its role as a human milk oligosaccharide (HMO). It is added to infant formula to mimic the composition of breast milk, promoting healthy gut microbiota development in infants. This compound supports the growth of beneficial bacteria like Bifidobacteria, enhancing digestive health and immune function. Additionally, it may help reduce the risk of infections by acting as a decoy receptor for pathogens, preventing them from binding to the intestinal lining. Its application extends to research in understanding gut-brain axis interactions and potential therapeutic uses in managing gastrointestinal disorders.