Triethylammonium bicarbonate buffer is widely used in biochemistry and molecular biology for its compatibility with mass spectrometry. It is particularly effective in the purification and analysis of peptides, proteins, and oligonucleotides, as it is volatile and can be easily removed during sample preparation. This buffer is commonly employed in ion-pairing reversed-phase chromatography to separate and purify nucleic acids, ensuring high recovery and minimal interference. Additionally, it is utilized in enzymatic reactions and protein digestion processes, where its mild pH and non-denaturing properties are advantageous. Its volatility makes it a preferred choice for applications requiring sample concentration or lyophilization.