GW3965 is primarily utilized in research settings as a potent and selective agonist for the liver X receptor (LXR). It is widely used to study the role of LXR in various biological processes, including lipid metabolism, inflammation, and cholesterol homeostasis. In experimental models, GW3965 has been shown to promote reverse cholesterol transport, making it a valuable tool for investigating potential therapeutic strategies for atherosclerosis and other cardiovascular diseases. Additionally, it is employed in studies exploring its effects on immune responses, neuroinflammation, and cancer, particularly in understanding how LXR activation influences tumor growth and metastasis. Its application extends to preclinical research aimed at developing LXR-targeted therapies for metabolic and inflammatory disorders.