Used in cardiovascular research to study endothelial function and vascular tone regulation. Serves as a precursor to endothelin-1, a potent vasoconstrictor, allowing scientists to investigate mechanisms of blood pressure control and endothelial cell behavior. Applied in assays to measure endothelin-converting enzyme activity and to explore pathophysiological conditions such as hypertension, heart failure, and pulmonary arterial hypertension. Also utilized in receptor binding studies to examine endothelin receptor subtypes (ETA and ETB) and their signaling pathways. Valuable tool in developing and testing inhibitors targeting the endothelin system.