Used in research to inhibit protein arginine methyltransferases (PRMTs), particularly PRMT1. This inhibition helps study the role of arginine methylation in cellular processes such as gene expression, signal transduction, and RNA processing. AMI-1 is valuable in exploring epigenetic regulation and has been applied in studies related to cancer, inflammation, and viral replication due to its ability to modulate PRMT activity. It serves as a tool compound for understanding disease mechanisms and assessing the therapeutic potential of targeting PRMT enzymes.