CARM1-IN-1 is primarily used in research settings to study the role of CARM1 (coactivator-associated arginine methyltransferase 1) in various biological processes. It acts as an inhibitor of CARM1, which is involved in the methylation of proteins, including histones, thereby influencing gene expression. Researchers utilize this compound to investigate its potential in modulating epigenetic regulation, particularly in cancer biology. By inhibiting CARM1, it helps in understanding the enzyme's contribution to tumor growth and progression, offering insights into potential therapeutic strategies for cancer treatment. Additionally, CARM1-IN-1 is employed in studies related to stem cell differentiation and immune response, as CARM1 plays a role in these processes. Its application extends to exploring its effects on metabolic pathways and cellular signaling mechanisms, contributing to the broader understanding of epigenetic modifications in disease and development.