AMI-1 is primarily utilized in biochemical research as a potent and selective inhibitor of protein arginine methyltransferases (PRMTs). These enzymes play a crucial role in the methylation of arginine residues in proteins, which is a key post-translational modification involved in various cellular processes. By inhibiting PRMTs, AMI-1 helps researchers study the effects of arginine methylation on gene expression, signal transduction, and protein function. This makes it a valuable tool in understanding the molecular mechanisms underlying diseases such as cancer, where abnormal protein methylation is often observed. Additionally, AMI-1 is used in the development of therapeutic strategies targeting PRMTs, offering potential avenues for the treatment of conditions linked to dysregulated methylation.