BIX-01338 hydrate is primarily utilized in biochemical research as a potent and selective inhibitor of the G9a histone methyltransferase. It plays a significant role in studying epigenetic regulation, particularly in understanding the mechanisms of histone methylation and its impact on gene expression. Researchers employ this compound to explore cellular processes such as differentiation, proliferation, and reprogramming, especially in stem cell biology. Its ability to modulate histone methylation makes it a valuable tool in investigating potential therapeutic strategies for diseases linked to epigenetic dysregulation, including cancer and neurological disorders. Additionally, BIX-01338 hydrate is used in experimental studies to assess its effects on chromatin structure and function, contributing to advancements in epigenetics and drug discovery.