SGC-0946 is primarily used in biochemical research as a potent and selective inhibitor of the histone methyltransferase enzyme DOT1L. It plays a significant role in studying the biological functions of DOT1L, particularly in the context of gene regulation and chromatin remodeling. Researchers utilize this compound to investigate its effects on transcription processes and its potential implications in diseases such as leukemia, where DOT1L activity is often dysregulated. Additionally, SGC-0946 serves as a valuable tool in exploring therapeutic strategies targeting epigenetic modifications in cancer and other disorders linked to aberrant histone methylation.