EPZ020411 2HCl is primarily utilized in the field of biomedical research, particularly in the study of epigenetic regulation. It serves as a potent and selective inhibitor of the protein arginine methyltransferase 4 (PRMT4), also known as coactivator-associated arginine methyltransferase 1 (CARM1). This compound is widely used to investigate the role of CARM1 in various cellular processes, including gene transcription, cell differentiation, and cancer progression. By inhibiting CARM1, researchers can better understand its function in disease mechanisms, particularly in cancers where CARM1 overexpression is observed. This makes it a valuable tool for developing potential therapeutic strategies targeting epigenetic modifications in oncology and other areas of molecular biology.