KDM5-IN-1 is primarily used in research settings to inhibit the activity of KDM5 histone demethylases, which are enzymes involved in the regulation of gene expression through histone modification. By blocking these enzymes, it helps scientists study the role of KDM5 in various biological processes, including cancer development and stem cell differentiation. Its application is particularly significant in understanding epigenetic mechanisms and exploring potential therapeutic strategies for diseases linked to abnormal histone methylation, such as certain cancers. Researchers also use it to investigate how KDM5 inhibition affects cell proliferation, apoptosis, and response to other treatments.