ML324 is primarily used in research settings to study its effects on cancer cells, particularly in the context of inhibiting the JMJD2 demethylase enzyme. This inhibition is crucial for understanding epigenetic regulation and its role in cancer progression. Researchers utilize ML324 to explore potential therapeutic strategies for various cancers by targeting epigenetic modifications. Additionally, it is employed in studies focusing on cellular differentiation and proliferation, providing insights into how epigenetic mechanisms influence these processes. The compound is also valuable in chemical biology for probing the function of histone demethylases in disease models.