Used as a biological stain in microbiology to differentiate bacterial species, particularly in Gram staining, where it helps identify Gram-positive bacteria by binding to their cell walls. It is also employed in histology to stain tissues for microscopic examination, enhancing the visibility of cellular structures. In addition, it serves as a pH indicator, changing color in response to acidity or alkalinity. The dye is utilized in the textile industry for coloring fabrics and in the production of inks and paints. Furthermore, it has applications in research as a model compound for studying dye adsorption and degradation processes.