This chemical is primarily used as a biological stain in microscopy and histology. It is particularly effective in staining tissues and cells for detailed examination under a microscope. The compound is also employed in the textile industry as a dye for fabrics, providing a vibrant blue color. Additionally, it serves as an indicator in certain chemical reactions due to its distinct color change properties. In research, it is utilized to study oxidative stress and cellular responses, making it a valuable tool in biochemical and medical studies. Its water-soluble nature ensures ease of use in various aqueous applications.