Methyl Green is widely used as a biological stain in microscopy, particularly for highlighting nucleic acids like DNA in cell nuclei. It is often employed in histology to differentiate chromatin and chromosomes, providing clear visualization under a microscope. The stain is also utilized in electrophoresis to track DNA fragments due to its binding affinity. In addition, Methyl Green finds application in certain biochemical assays to study enzyme activity, especially those involving nucleic acids. Its ability to selectively stain specific cellular components makes it a valuable tool in both research and diagnostic laboratories.