Mordant Orange 1 is primarily used as a pH indicator with a transition interval from yellow at pH 10.0 to red at pH 12.0, commonly employed in analytical chemistry and laboratory titrations. It is also utilized as a mordant dye in the textile industry, particularly for coloring wool, silk, and nylon, where its ability to form stable complexes with metal ions enables vibrant and durable shades in mordant dyeing processes. Additionally, the dye finds application in biological staining techniques to visualize tissues and cellular structures under a microscope, and in the leather industry for dyeing leather products to achieve long-lasting colors. Its versatility as both an indicator and dye makes it valuable in various industrial and scientific applications.