Chromotrope 2B is primarily used as a biological stain in histology and microscopy, particularly for staining connective tissues and elastic fibers, enabling clear visualization under a microscope. It is also applied in microbiological research for staining cellular structures. In the textile industry, it serves as a dye for natural and synthetic fabrics, including cotton and linen, providing vibrant red to purple hues that are durable. Additionally, it is used in the paper industry for dyeing paper products, ensuring consistent and long-lasting color. As a pH indicator in analytical chemistry, it detects changes in acidity or basicity and specific substances in reactions. Its versatility makes it valuable in scientific and industrial applications.