Used primarily in histology, this stain is valuable for differentiating various tissue components in pathological examinations. It effectively highlights five key elements: collagen appears yellow, elastic fibers black, fibrin and cytoplasm red, muscle fibers red or deep pink, and mucin blue or green. This multi-color contrast makes it especially useful in studying complex tissue architectures, such as those found in blood vessels and heart tissues. It is commonly applied in research and diagnostics of cardiovascular diseases, vasculitis, and fibrotic conditions, where clear visualization of extracellular matrix components is critical. The stain is also employed in toxicology and animal studies to assess tissue damage and repair mechanisms.