Used in histology for staining tissue sections, particularly effective in differentiating cell structures such as nuclei and cytoplasm. Commonly applied to highlight striated muscle fibers, allowing pathologists to identify cross-striations in cardiac and skeletal muscle tissues. Also used to stain fibrin in blood clots, aiding in the diagnosis of thrombotic conditions. The stain provides contrast between collagen and muscle, supporting evaluation of tissue architecture in tumors and inflammatory lesions. Frequently utilized in central nervous system studies to visualize glial fibers and neurofibrillary tangles. Its ability to bind phosphorus-rich compounds makes it valuable for demonstrating mitochondria and certain epithelial cells. Often part of special staining panels in diagnostic pathology and research laboratories.