Used in histology to differentiate and visualize various tissue components under the microscope. Masson's trichrome stain colors collagen fibers blue, muscle fibers and cytoplasm red, and nuclei black or dark blue, allowing clear distinction between connective tissue and muscle tissue. Commonly applied in the study of cardiac and skeletal muscle pathologies, liver diseases, and fibrosis assessment. Helps in identifying structural changes in tissues, such as fibrosis or necrosis, making it valuable in diagnostic and research pathology.