Used in histopathology to differentiate various tissue components under the microscope. Stains collagen fibers blue, muscle fibers red, and nuclei dark brown or black. Helps in the assessment of fibrosis in liver, heart, and kidney tissues. Commonly applied in research and diagnostic labs to evaluate tissue architecture and pathological changes such as cirrhosis or myocardial damage. Provides clear contrast between connective tissue and muscle, aiding in the identification of tumors and structural abnormalities. Suitable for use on formalin-fixed, paraffin-embedded tissue sections.