Used primarily in histology for staining myelin sheaths in nervous tissue sections. It helps visualize the integrity and distribution of myelin in brain and spinal cord samples, making it valuable in diagnosing demyelinating diseases such as multiple sclerosis. The stain produces a bright blue color in myelinated areas, allowing clear contrast under the microscope. Often combined with a counterstain like cresyl violet or neutral red to highlight cell nuclei or other structures. Commonly used in both research and clinical pathology settings for neuroanatomical studies and neuropathological diagnosis.