Used in histopathology to visualize reticular fibers in tissue sections, particularly in liver, spleen, and bone marrow biopsies. The stain helps assess structural integrity and architectural patterns in these tissues, aiding in the diagnosis of conditions such as cirrhosis, tumors, and fibrosis. It is commonly applied in routine diagnostic workflows to differentiate reticular fiber networks from collagen fibers, providing clarity in tissue morphology under microscopic examination.