Used in histology and pathology to detect polysaccharides such as glycogen, glycoproteins, and mucins in tissue sections. Commonly applied in identifying basement membranes, fungal cell walls, and glycogen deposits in liver tissues. Helps diagnose conditions like glycogen storage diseases, adenocarcinomas (which often produce mucins), and certain fungal infections. The stain turns positive structures magenta or purple-red, making them easily visible under a microscope. Widely used in clinical diagnostics and research laboratories for tissue analysis.