Used in histology and pathology laboratories to detect melanin pigment in tissue sections, particularly in diagnosing melanoma and other pigmented lesions. The kit employs the ferrous sulfate method to specifically stain melanin, making it visible under microscopic examination. It helps differentiate melanin from other pigments like hemosiderin or lipofuscin, which is critical in accurate tissue diagnosis. The staining technique is commonly applied to formalin-fixed, paraffin-embedded tissues and provides reliable results with minimal background interference. It is also useful in forensic and dermatological studies where pigment distribution needs to be analyzed.