Used in histology and neuroscience research to stain nervous tissue sections, allowing clear visualization of neuronal cell bodies and overall tissue architecture. It combines the selective staining of Nissl substance in neurons with counterstaining of the background, enhancing contrast for easier identification of cellular structures under light microscopy. Commonly applied in brain and spinal cord studies to assess neuronal health, detect pathological changes, and map neural regions in both normal and diseased states. Also used in educational settings for teaching neuroanatomy due to its reliability and clarity in showing neuronal morphology.