Phenosafranine is widely used as a biological stain in microscopy for differentiating tissues and cells, particularly in histology and cytology. It helps in visualizing cellular structures under a microscope. In the textile industry, it serves as a dye for coloring fabrics, especially cotton and silk, due to its strong affinity for these materials. Additionally, it is employed in analytical chemistry as a redox indicator in titration processes, aiding in determining the endpoint of reactions. Phenosafranine also finds application in photodynamic therapy research, where it is studied for its potential to generate reactive oxygen species under light exposure, targeting cancer cells. Its role in electrochemical studies is notable, as it is used to investigate electron transfer processes in various systems.