Fast Red ITR is widely used in biochemical and histochemical staining procedures. It is particularly effective as a chromogen in immunohistochemistry (IHC) for detecting antigens in tissue sections. When coupled with alkaline phosphatase, it produces a vivid red precipitate, making it ideal for visualizing specific proteins or cellular components under a microscope. This property is valuable in research and diagnostic labs for studying disease markers, tissue structures, and cellular processes. Additionally, it is employed in enzyme-linked immunosorbent assays (ELISAs) to detect and quantify target molecules in biological samples. Its stability and intense color output make it a preferred choice for applications requiring high sensitivity and clarity.