Evans Blue is widely used in biomedical research as a vital dye for assessing vascular permeability and blood-brain barrier integrity. It binds to serum albumin, allowing researchers to track and quantify the leakage of plasma proteins into tissues, which is particularly useful in studies involving inflammation, trauma, or disease. In neuroscience, it is employed to evaluate the extent of brain injury or disruption of the blood-brain barrier in experimental models. Additionally, Evans Blue is utilized in histology to stain tissues for microscopic examination, aiding in the visualization of vascular structures. Its application extends to pharmacokinetic studies, where it helps determine the distribution and clearance of drugs in the body.