Amido Black 10B is widely used in biological and biochemical research as a staining agent for proteins. It is particularly effective in visualizing protein bands on polyacrylamide gels after electrophoresis, providing a clear contrast for analysis. Additionally, it is employed in forensic science for enhancing the visibility of bloodstains on various surfaces, aiding in crime scene investigations. The dye’s strong affinity for proteins makes it a valuable tool in histology for tissue staining, enabling detailed microscopic examination. Its application extends to the textile industry as well, where it is used for dyeing fabrics, especially those made of natural fibers like cotton and wool.