Ruthenium Red is widely used in biological research as a staining agent to visualize calcium channels and calcium-binding proteins in cells. It helps in studying cellular processes involving calcium signaling, which is crucial for understanding muscle contraction, neurotransmitter release, and cell death mechanisms. Additionally, it is employed in electron microscopy to enhance the contrast of cellular structures, particularly in mitochondria and endoplasmic reticulum. In pharmacology, Ruthenium Red is utilized to investigate the role of calcium in various diseases, such as neurodegeneration and cardiac dysfunction, aiding in the development of targeted therapies. Its ability to block certain calcium channels also makes it a valuable tool in studying calcium-dependent pathways in cellular physiology.