Phenazine-1,6-diol is primarily utilized in the field of biochemistry and microbiology due to its redox-active properties. It serves as a crucial component in the study of electron transfer processes, often employed as a redox mediator in microbial fuel cells to enhance electron transfer efficiency between microorganisms and electrodes. Additionally, it is investigated for its potential antimicrobial activity, making it a candidate for developing new antibacterial agents. Its ability to generate reactive oxygen species also positions it as a useful tool in oxidative stress research, particularly in understanding cellular damage and defense mechanisms. Furthermore, it finds application in synthetic chemistry as a building block for the preparation of more complex phenazine derivatives, which are explored for their diverse biological activities.