N-Mal-n-bis(peg2-nhs ester) is widely used in bioconjugation and protein modification due to its reactive NHS ester and maleimide groups. It is particularly valuable in linking biomolecules, such as peptides, proteins, or antibodies, to other functional groups or surfaces. The NHS ester reacts with primary amines, while the maleimide group selectively binds to thiols, enabling precise and efficient crosslinking. This compound is commonly applied in drug delivery systems, where it facilitates the attachment of therapeutic agents to targeting molecules. Additionally, it plays a role in the development of diagnostic tools, such as fluorescent probes or biosensors, by enabling the conjugation of detection molecules to biomolecules. Its PEG spacer enhances solubility and reduces immunogenicity, making it suitable for in vivo applications.